SOLUBILITY OPTIMIZATION

Self-interaction chromatography (SIC) is a tool we have used for years to evaluate the strength of interactions between peptide and protein molecules.

(cf., Valente et al., Curr. Pharm. Biotechnol. 2005, 6: 427-436; Valente et al., Biophys. J. 2005, 89: 4211-4218; Payne et al., Biopolymers (Peptide Sci.) 2006, 84: 527-533).

The SIC experiment indicates the sign and magnitude of the second osmotic virial coefficient or B value. This physical parameter has been shown to correlate with solubility and aggregation propensity. As a result, we can identify the solution conditions that are most likely to produce maximal stability and solubility in a short time frame and with small amounts of material using standard HPLC equipment.